What’s the CELL HANDLER™?
The CELL HANDLER™ is an automated system for selecting and isolating spheroids/organoids or single cells individually.
The integration of sophisticated picking and imaging technology enables precise cell isolation that is unattainable by conventional methods.
The CELL HANDLER™ can enhance the efficiency of drug discovery and biomedical research through the expansion of options in cell-based screening, cell quality management and cell line development.
Ignites your R&D.
Enabling new possibilities
CELL HANDLER™ can lead to new discoveries by enabling cell isolation of individual cells, which is not feasible using conventional methods.
Saving time and effort
Through automation of the process from identification to isolation of target cells, the efficiency and reproducibility of research are improved.
Selected cells are carefully picked, transferred and dispensed by gentle pipetting.
Easy to use
The system consists of pipetting and microscopic technology, and is easy to set up.
Automatic data saving
All data (images, cell features, well positions, picking conditions, etc.,) are stored and are traceable for downstream analysis.
Incredibly reliable cell isolation
Multiple imaging mode
Equipped with x4 and x10 magnification lenses and three fluorescence colors that enables evaluation of cells by morphological & phenotypic traits.
Wide range of cell picking
Reliable cell isolation in a wide range of cell type, size and culture conditions including medium, culture plate, etc.,. The Precise control of 8 pipet heads affords isolation of randomly distributed cells in semi-solid or hydrogel media.
With intuitive software it allows easy designation of the number of transferring cells and target wells and offers customized designing of the assay plate. Detects the target cells at high speed automatically by utilizing an advanced image processing algorithm.
Clean working environment
The enclosed workspace contains an automatically controlled door and equips HEPA filters and UV lamps.
Supports various applications
Allows the use of limited amounts of valuable samples in cell-based assays, such as drug sensitivity testing.
Stem cell research
Capable of isolating various types of cells, such as iPSC, ES and adult stem cell in a damage-free manner.
Supports research using spheroids, organoids and ex vivo tissues.
Toxicology / Oncology Research
Supports accurate placement of cells on specialized plates such as Organ-on-a-Chip.
Single cell research
Enables single cell isolation for gene expression and transcriptome analysis.
Cell line establishment /
Antibody-producing cell screening
Identifies high antibody producing cells and isolates clonal cell lines.
Simple and flexible 4-step process supports new discoveries
- STEP1: Detection
Recognizes a wide range of cell types and sizes and measures distinct morphological & phenotypic features.
Is compatible with various source plates.
1) Advanced image processing technology
Morphological & phenotypic features of cells in the source plate are obtained by high-throughput image analysis.
Detection of 3D-cell aggregates
Detection of single cells
2) Various source plates
A wide range of 3D culture plates are supported in addition to SBS format microplates and petri dishes.
Example of 3D culture plates (microcavity, nanowell plate, etc.,)
*1 “CellTracker” is a trade mark of Thermo Fischer Scientific Inc..
*2 “Elplasia” is a registered trademark of Corning Inc..
*3 “AggreWell” is a trademark of STEMCELL Technologies Inc..
*4 “Cell-able” is a registered trademark of TOYO GOSEI CO., LTD . in Japan and in the US.
- STEP2: Selection
Select target cells using acquired cellular parameters.
There are two modes for cell selection, automatic and manual.
1) Automatic selection (Histogram selection)
Cellular features (20 distinct parameters) are instantly visualized in a histogram. By combining multiple features and threshold limits as selection criteria, a target group of cells can be automatically selected.
2) Manual selection (Machine learning)
Users can manually select the desired cells. When manual selection is used, the machine learning function can be activated to learn the researcher preferences and to instruct automatic selection of the optimal cell group after iteration.
- STEP3: Handling
Selected cells are picked and dispensed in a damage-free manner using gentle pipetting action.
They can be isolated from liquid medium as well as hydrogels or solid matrices.
1) Flexible size compatibility
Compatible with single cell size up to 180 μm spheroids and organoids.
(Please contact us for support for larger cells (<2 mm).)
- Precision Tip™
- Inner dia. Φ180 µm
- Positive displacement
2) High-speed isolation with 8 heads
Precisely controlled 8 pipet heads aspirate and dispense cells in a gentle, but high-throughput manner.
Transfer to a 384-well plate: 30 minutes to transfer 384 cells/spheroids and to obtain images of all the wells.
3) Various destination plates
In addition to the SBS format plates, it is also possible to transfer cells to other designation sources such as PCR tubes and an Organ-on-a-Chip plate.
From 6 to 384-well plates
OrganoPlate® Graft *5
*5 “OrganoPlate” is a registered trademark of Mimetas BV.
- STEP4: Verification
Successful cell isolation is confirmed by imaging the destination plate.
Verification of process completion is possible by imaging the source plate before and after picking.
Image of a whole 384-well plate
1) Confirmation of cell isolation by imaging
Accurate cell isolation can be confirmed by comparing images from the source plate before and after picking and the destination plate after dispensing. This is an effective way to confirm monoclonality with traceability.
Example of isolation from hydrogel dome
Source plate (6-well)
Destination plate (384-well)
2) Data output traceability
All acquired data (i.e. images, cell features, well positions) and settings are automatically tracked throughout the process.
*For research use only. Not for use in diagnostic or therapeutic procedures.
*The specifications are subject to change without notice.
*The above are the results of experiments in our laboratory. The results may vary depending on the work environment, cell type and so on.