Selection of transfectants (fluorescent clones)
Please see the following Application examples.
CHO cells were transfected with a GFP expression vector containing a puromycin-resistance marker.
After selection by puromycin for 8 days, surviving cells were dispensed on a Elplasia™* plate.
Single cells were detected by brightfield and fluorescence imaging (Fig. 1).
CELL HANDLER™ picked one of the fluorescent target cells and transferred it to a flat bottom plate.
Six days after transfer, a colony was formed with stable fluorescence, showing the ability of CELL HANDLER™ in accurately picking single-cell transfectants. Also, CELL HANDLER™ helps save time and effort in establishing stable clones and determining their monoclonality.
Tiled images are shown in Figure 2.
* “Elplasia” is a trademark of Kuraray Corporation.
* For research use only. Not for use in diagnostic or therapeutic procedures.
* The specifications are subject to change without notice.
* The above are the results of experiments in our laboratory. The results may vary depending on the work environment, cell type and so on.