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The Cell picking & imaging system. CELL HANDLER™

Selection of transfectants (fluorescent clones) / Imaging options for single cell

Please see the following Application examples.

Selection of transfectants (fluorescent clones)

CHO cells were transfected with a GFP expression vector containing a puromycin-resistance marker. After selection by puromycin for 8 days, surviving cells were dispensed on a Elplasia® plate. Single cells were detected by bright-field and fluorescence imaging with 4x magnification (Fig. 1). CELL HANDLER™ picked one of the fluorescent target cells and transferred it to a flat bottom plate. Six days after transfer, a colony was formed with stable fluorescence, showing the ability of CELL HANDLER™ in accurately picking single-cell transfectants. Tiled images that cover the whole bottom of a well are shown in Figure 1.

CELL HANDLER™ saves time and effort in isolating and establishing stable-transfectant monoclonal cell lines.

Figure 1

Imaging options for single cell

CELL HANDLER™ has imaging capabilities with 10X magnification and multi-color fluorescent detection.Comparison of 4X and 10X (Fig. 2 left) and 2 different colors detection (Fig. 2 right) were shown.

Figure 2

* "CellTracker" is a trade mark of Thermo Fischer Scientific Inc.. * "Elplasia" is a registered trademark of Corning Inc..

* For research use only. Not for use in diagnostic or therapeutic procedures.
* The specifications are subject to change without notice.
* The above are the results of experiments in our laboratory. The results may vary depending on the work environment, cell type and so on.

*Please note that we will not be able to respond to inquiries about anything other than CELL HANDLER™.
*Please note that it may take some time to response to certain inquiries.

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